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KRT20 Rabbit pAb

Anti-KRT20 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221168

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221168
Product Name:   Anti-KRT20 Rabbit Polyclonal Antibody
Synonyms:   K20; CD20; CK20; CK-20; KRT21
UNIPROT ID:   P35900 (Gene Accession – NP_061883 )
Background:    The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.
Immunogen:    Synthetic peptide of human KRT20
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 221168(KRT20 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the synthetic peptide and then with 221168(Anti-KRT20 Antibody) at dilution 1/20.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: LOVO cell and Mouse small intestines tissue lysates;
Primary antibody: 221168(KRT20 Antibody) at dilution 1/600;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 minutes
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