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ATF1 Rabbit pAb

Anti-ATF1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221178

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221178
Product Name:   Anti-ATF1 Rabbit Polyclonal Antibody
Synonyms:   TREB36; EWS-ATF1; FUS/ATF-1
UNIPROT ID:   P18846 (Gene Accession – NP_005162 )
Background:    This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chromosome 6.
Immunogen:    Synthetic peptide of human ATF1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 221178(ATF1 Antibody) at a dilution of 1/35(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the synthetic peptide and then with 221178(Anti-ATF1 Antibody) at dilution 1/35.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 221178(Anti-ATF1 Antibody) at a dilution of 1/35.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with synthetic peptide and then with D262664(Anti-ATF1 Antibody) at dilution 1/35.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: Human placenta tissue lysate;
Primary antibody: 221178(ATF1 Antibody) at dilution 1/600;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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