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CLDN15 Rabbit pAb

Anti-CLDN15 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221193

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221193
Product Name:   Anti-CLDN15 Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   P56746 (Gene Accession – NP_055158 )
Background:    This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jun 2010]
Immunogen:    Synthetic peptide of human CLDN15
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 221193(CLDN15 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 221193(Anti-CLDN15 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using 221193(Anti-CLDN15 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with synthetic peptide and then with D262689(Anti-CLDN15 Antibody) at dilution 1/50.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: THP-1 cell lysate;
Primary antibody: 221193(CLDN15 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 40 seconds
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