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PRDX4 Rabbit pAb

Anti-PRDX4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221294

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221294
Product Name:   Anti-PRDX4 Rabbit Polyclonal Antibody
Synonyms:   PRX-4; AOE372; AOE37-2; HEL-S-97n
UNIPROT ID:   Q13162 (Gene Accession – NP_006397 )
Background:    The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB.
Immunogen:    Synthetic peptide of human PRDX4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 221294(PRDX4 Antibody) at a dilution of 1/25(Cytoplasm and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the synthetic peptide and then with 221294(Anti-PRDX4 Antibody) at dilution 1/25.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-6: MCF-7, K562, HepG2, 293T, Raji and Hela cell;
Primary antibody: 221294(PRDX4 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 4 seconds
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