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IRF5 Rabbit pAb

Anti-IRF5 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221448

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221448
Product Name:   Anti-IRF5 Rabbit Polyclonal Antibody
Synonyms:   SLEB10
UNIPROT ID:   Q13568 (Gene Accession – NP_116032 )
Background:    This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Multiple transcript variants encoding different isoforms have been found for this gene, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment.
Immunogen:    Synthetic peptide of human IRF5
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 221448(IRF5 Antibody) at a dilution of 1/25(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the synthetic peptide and then with 221448(Anti-IRF5 Antibody) at dilution 1/25.

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 221448(Anti-IRF5 Antibody) at a dilution of 1/25.

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with synthetic peptide and then with D263043(Anti-IRF5 Antibody) at dilution 1/25.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: NIH/3T3, Hela, Jurkat, Hepg2 and A549 cell lysates;
Primary antibody: 221448(IRF5 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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