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PRKACA Rabbit pAb

Anti-PRKACA Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221674

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221674
Product Name:   Anti-PRKACA Rabbit Polyclonal Antibody
Synonyms:   PKACA; PPNAD4
UNIPROT ID:   P17612 (Gene Accession – NP_002721 )
Background:    This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing’s syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms.
Immunogen:    Synthetic peptide of human PRKACA
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer, Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 221674(PRKACA Antibody) at a dilution of 1/30(Cytoplasm or Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 221674(Anti-PRKACA Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 221674(Anti-PRKACA Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with synthetic peptide and then with D263374(Anti-PRKACA Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Human testis tissue lysate;
Primary antibody: 221674(PRKACA Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 seconds
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