Cat.#: S221761 |
Product Name: Anti-CARD16 Rabbit Polyclonal Antibody |
Synonyms: COP; COP1; PSEUDO-ICE |
UNIPROT ID: Q5EG05 (Gene Accession – NP_001017534 ) |
Background: Caspase inhibitor. Acts as a regulator of procaspase-1/CASP1 activation implicated in the regulation of the proteolytic maturation of pro-interleukin-1 beta (IL1B) and its release during inflammation. Inhibits the release of IL1B in response to LPS in monocytes. Also induces NF-kappa-B activation during the pro-inflammatory cytokine response. Also able to inhibit CASP1-mediated neuronal cell death, TNF-alpha, hypoxia-, UV-, and staurosporine-mediated cell death but not ER stress-mediated cell death. Acts by preventing activation of caspases CASP1 and CASP4, possibly by preventing the interaction between CASP1 and RIPK2. |
Immunogen: Synthetic peptide of human CARD16 |
Applications: ELISA, WB, IHC |
Recommended Dilutions: IHC: 25-100;WB: 200-1000;ELISA: 5000-10000 |
Host Species: Rabbit |
Clonality: Rabbit Polyclonal |
Isotype: Immunogen-specific rabbit IgG |
Purification: Antigen affinity purification |
Species Reactivity: Human |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
Research Areas: Cancer |
Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing |
Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 221761(CARD16 Antibody) at a dilution of 1/25(Cytoplasm). | In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 221761(Anti-CARD16 Antibody) at dilution 1/25. | The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 221761(Anti-CARD16 Antibody) at a dilution of 1/25. | In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with synthetic peptide and then with D263494(Anti-CARD16 Antibody) at dilution 1/25. | Gel: 12%SDS-PAGE, Lysate: 40 μg; Lane: HEPG2 cell lysate; Primary antibody: 221761(CARD16 Antibody) at dilution 1/400; Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution; Exposure time: 60 seconds | |
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