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MBD2-b Rabbit pAb

Anti-MBD2-b Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221794

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221794
Product Name:   Anti-MBD2-b Rabbit Polyclonal Antibody
Synonyms:   DMTase; NY-CO-41
UNIPROT ID:   Q9UBB5 (Gene Accession – NP_056647 )
Background:    DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Synthetic peptide of human MBD2-b
Applications:    ELISA, IHC
Recommended Dilutions:    IHC: 20-100; ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 221794(MBD2-b Antibody) at a dilution of 1/20(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the synthetic peptide and then with 221794(Anti-MBD2-b Antibody) at dilution 1/20.
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