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MAP1LC3B Rabbit pAb

Anti-MAP1LC3B Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221803

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221803
Product Name:   Anti-MAP1LC3B Rabbit Polyclonal Antibody
Synonyms:   LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
UNIPROT ID:   Q9GZQ8 (Gene Accession – NP_073729 )
Background:    The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Immunogen:    Synthetic peptide of human MAP1LC3B
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 20-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer, Neuroscience, Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 221803(MAP1LC3B Antibody) at a dilution of 1/25(Cytoplasm or Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 221803(Anti-MAP1LC3B Antibody) at dilution 1/25.

The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using 221803(Anti-MAP1LC3B Antibody) at a dilution of 1/25.

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with synthetic peptide and then with D263557(Anti-MAP1LC3B Antibody) at dilution 1/25.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: HEPG2 cell and Human fetal brain tissue lysates;
Primary antibody: 221803(MAP1LC3B Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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