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MARK2 Rabbit pAb

Anti-MARK2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221826

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221826
Product Name:   Anti-MARK2 Rabbit Polyclonal Antibody
Synonyms:   EMK1; EMK-1; PAR-1; Par1b; Par-1b
UNIPROT ID:   Q7KZI7 (Gene Accession – NP_001034558 )
Background:    This gene encodes a member of the Par-1 family of serine/threonine protein kinases. The protein is an important regulator of cell polarity in epithelial and neuronal cells, and also controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes. Multiple transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Synthetic peptide of human MARK2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: Oct-50;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 221826(MARK2 Antibody) at a dilution of 1/25(Cytoplasm and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 221826(Anti-MARK2 Antibody) at dilution 1/25.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: A431 and Raji cell lysates;
Primary antibody: 221826(MARK2 Antibody) at dilution 1/550;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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