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MSH2 Rabbit pAb

Anti-MSH2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221854

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221854
Product Name:   Anti-MSH2 Rabbit Polyclonal Antibody
Synonyms:   FCC1; COCA1; HNPCC; LCFS2; HNPCC1
UNIPROT ID:   P43246 (Gene Accession – NP_000242 )
Background:    This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Synthetic peptide of human MSH2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 20-100;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 221854(MSH2 Antibody) at a dilution of 1/20(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the synthetic peptide and then with 221854(Anti-MSH2 Antibody) at dilution 1/20.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: HEPG2, 293T, SKOV3, Hela and A172 cell lysates;
Primary antibody: 221854(MSH2 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
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