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GTF3A Rabbit pAb

Anti-GTF3A Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S222152

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S222152
Product Name:   Anti-GTF3A Rabbit Polyclonal Antibody
Synonyms:   AP2; TFIIIA
UNIPROT ID:   Q92664 (Gene Accession – NP_002088 )
Background:    The product of this gene is a zinc finger protein with nine Cis[2]-His[2] zinc finger domains. It functions as an RNA polymerase III transcription factor to induce transcription of the 5S rRNA genes. The protein binds to a 50 bp internal promoter in the 5S genes called the internal control region (ICR), and nucleates formation of a stable preinitiation complex. This complex recruits the TFIIIC and TFIIIB transcription factors and RNA polymerase III to form the complete transcription complex. The protein is thought to be translated using a non-AUG translation initiation site in mammals based on sequence analysis, protein homology, and the size of the purified protein.
Immunogen:    Synthetic peptide of human GTF3A
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 222152(GTF3A Antibody) at a dilution of 1/60(Nucleus and Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the synthetic peptide and then with 222152(Anti-GTF3A Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 222152(Anti-GTF3A Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with synthetic peptide and then with D264139(Anti-GTF3A Antibody) at dilution 1/60.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: TM4 cell lysate;
Primary antibody: 222152(GTF3A Antibody) at dilution 1/550;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 7 seconds
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