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GTF3C4 Rabbit pAb

Anti-GTF3C4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S222154

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S222154
Product Name:   Anti-GTF3C4 Rabbit Polyclonal Antibody
Synonyms:   KAT12; TFIII90; TFIIIC90; TFIIIC290; TF3C-delta; TFIIICDELTA
UNIPROT ID:   Q9UKN8 (Gene Accession – NP_036336 )
Background:    Essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3. May cooperate with GTF3C5 in facilitating the recruitment of TFIIIB and RNA polymerase through direct interactions with BRF1, POLR3C and POLR3F. May be localized close to the A box.
Immunogen:    Synthetic peptide of human GTF3C4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 150-300;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 222154(GTF3C4 Antibody) at a dilution of 1/160(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the synthetic peptide and then with 222154(Anti-GTF3C4 Antibody) at dilution 1/160.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: A172, Hela, Jurkat and Raji cell lysates;
Primary antibody: 222154(GTF3C4 Antibody) at dilution 1/1300;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 2 minutes
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