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HLA-DRB1 Rabbit pAb

Anti-HLA-DRB1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S222185

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S222185
Product Name:   Anti-HLA-DRB1 Rabbit Polyclonal Antibody
Synonyms:   SS1; DRB1; HLA-DRB; HLA-DR1B
UNIPROT ID:   P20039 (Gene Accession – Hs.534322 )
Background:    HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4 and DRB5. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.
Immunogen:    Synthetic peptide of human HLA-DRB1
Applications:    ELISA, IHC
Recommended Dilutions:    IHC: 20-100; ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 222185(HLA-DRB1 Antibody) at a dilution of 1/35(Cytoplasm and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the synthetic peptide and then with 222185(Anti-HLA-DRB1 Antibody) at dilution 1/35.
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