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HLA-DRB3 Rabbit pAb

Anti-HLA-DRB3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S222186

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S222186
Product Name:   Anti-HLA-DRB3 Rabbit Polyclonal Antibody
Synonyms:   HLA-DR1B; HLA-DR3B
UNIPROT ID:   P79483 (Gene Accession – NP_072049 )
Background:    HLA-DRB3 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB3 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.
Immunogen:    Synthetic peptide of human HLA-DRB3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: Oct-50;WB: 300-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 222186(HLA-DRB3 Antibody) at a dilution of 1/25(Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the synthetic peptide and then with 222186(Anti-HLA-DRB3 Antibody) at dilution 1/25.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: Raji cell lysate;
Primary antibody: 222186(HLA-DRB3 Antibody) at dilution 1/320;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 minutes
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