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ISY1-RAB43 Rabbit pAb

Anti-ISY1-RAB43 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S222253

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S222253
Product Name:   Anti-ISY1-RAB43 Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   Q9ULR0 (Gene Accession – NP_001191819 )
Background:    This locus represents naturally occurring read-through transcription between the neighboring ISY1 (ISY1 splicing factor homolog) and RAB43 (RAB43, member RAS oncogene family) gene on chromosome 3. The read-through transcript encodes a protein that shares sequence identity with the upstream gene product, but its C-terminus is distinct due to a frameshift relative to the downstream gene.
Immunogen:    Synthetic peptide of human ISY1-RAB43
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 40-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 222253(ISY1-RAB43 Antibody) at a dilution of 1/30(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the synthetic peptide and then with 222253(Anti-ISY1-RAB43 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 222253(Anti-ISY1-RAB43 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with synthetic peptide and then with D264282(Anti-ISY1-RAB43 Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: 293T, HepG2 and SKOV3 cell lysates;
Primary antibody: 222253(ISY1-RAB43 Antibody) at dilution 1/600;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 90 seconds
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