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PUM3 Rabbit pAb

Anti-PUM3 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S222299

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S222299
Product Name:   Anti-PUM3 Rabbit Polyclonal Antibody
Synonyms:   PEN; HA-8; PUF6; XTP5; PUF-A; HLA-HA8; KIAA0020
UNIPROT ID:   Q15397 (Gene Accession – NP_055693 )
Background:    Inhibits the poly(ADP-ribosyl)ation activity of PARP1 and the degradation of PARP1 by CASP3 following genotoxic stress (PubMed:21266351). Binds to double-stranded RNA or DNA without sequence specificity (PubMed:25512524). Involved in development of the eye and of primordial germ cells (By similarity).
Immunogen:    Synthetic peptide of human PUM3
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 222299(PUM3 Antibody) at a dilution of 1/80(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the synthetic peptide and then with 222299(Anti-PUM3 Antibody) at dilution 1/80.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Raji cell lysate ;
Primary antibody: 222299(PUM3 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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