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Spink1 Rabbit pAb

Anti-Spink1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S222322

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S222322
Product Name:   Anti-Spink1 Rabbit Polyclonal Antibody
Synonyms:   Tilp; Psti-1; Spink1
UNIPROT ID:   P09655 (Gene Accession – NP_036806 )
Background:    acts as a trypsin inhibitor to protect the pancreas from premature activation of pancreatic juice; has additional monitor peptide activity to induce cholecystokinin release in the intestine.
Immunogen:    Synthetic peptide of Rat Spink1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-50;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colorectal cancer tissue using 222322(Spink1 Antibody) at a dilution of 1/20(Secreted).

In comparision with the IHC on the left, the same paraffin-embedded Human colorectal cancer tissue is first treated with the synthetic peptide and then with 222322(Anti-Spink1 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 222322(Anti-Spink1 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with synthetic peptide and then with D264388(Anti-Spink1 Antibody) at dilution 1/20.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: Rat pancreas tissue lysate;
Primary antibody: 222322(Spink1 Antibody) at dilution 1/400;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 4 minutes
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