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S100A8 Rabbit pAb

Anti-S100A8 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S222415

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S222415
Product Name:   Anti-S100A8 Rabbit Polyclonal Antibody
Synonyms:   P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG; S100-A8
UNIPROT ID:   P05109 (Gene Accession – NP_002955 )
Background:    The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. Multiple transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Synthetic peptide of human S100A8
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Neuroscience, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 222415(S100A8 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the synthetic peptide and then with 222415(Anti-S100A8 Antibody) at dilution 1/50.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: HL-60 cell lysate;
Primary antibody: 222415(S100A8 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
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