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CCT7 Rabbit pAb

Anti-CCT7 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S222439

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S222439
Product Name:   Anti-CCT7 Rabbit Polyclonal Antibody
Synonyms:   CCTH; CCTETA; NIP7-1; TCP1ETA
UNIPROT ID:   Q99832 (Gene Accession – NP_006420 )
Background:    This gene encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 6.
Immunogen:    Synthetic peptide of human CCT7
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 222439(CCT7 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 222439(Anti-CCT7 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using 222439(Anti-CCT7 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with synthetic peptide and then with D264610(Anti-CCT7 Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-6: LNCAP, HepG2, Jurkat, K562, 293T, Hela cell lysates;
Primary antibody: 222439(CCT7 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 10 seconds
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