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KDM1A (1E5) Mouse mAb

Anti-KDM1A (1E5) Mouse Monoclonal Antibody
5/5

$349.00

Cat.#:  N261003

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N261003
Product Name:   Anti-KDM1A (1E5) Mouse Monoclonal Antibody
Synonyms:   KDM1A; AOF2; KDM1; KIAA0601; LSD1; Lysine-specific histone demethylase 1A; BRAF35-HDAC complex protein BHC110; Flavin-containing amine oxidase domain-containing protein 2
UNIPROT ID:   O60341
Background:    Histone demethylase that demethylates both ‘Lys-4’ (H3K4me) and ‘Lys-9’ (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes.
Immunogen:    Purified recombinant human KDM1/LSD1 protein fragments expressed in E.coli.
Applications:    WB,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    1E5-H2
MW:    Calculated MW: 93 kDa; Observed MW: 110 kDa
Isotype:    IgG1
Purification:    Affinity Purified
Species Reactivity:    Human,Monkey
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    EpigeneticsyyyyyHistone methylation
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunocytochemistry analysis of KDM1A (1E5) in HeLa using KDM1/LSD1 antibody.

Western blot analysis of KDM1/LSD1 in Hela, HCT116 and COS7 lysates using KDM1/LSD1 antibody.

Immunoprecipitation analysis of KDM1A (1E5) in Hela lysates using KDM1/LSD1 antibody.
          Publications