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Smad5 (4B10) Mouse mAb

Anti-Smad5 (4B10) Mouse Monoclonal Antibody
5/5

$349.00

Cat.#:  N261030

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N261030
Product Name:   Anti-Smad5 (4B10) Mouse Monoclonal Antibody
Synonyms:   DKFZp781C1895; DKFZp781O1323; Dwfc; hSmad 5; hSmad5; JV5 1; JV5-1; MAD homolog 5; MAD mothers against decapentaplegic homolog 5; MAD; mothers against decapentaplegic homolog 5; MADH 5; MADH5; Mothers against decapentaplegic homolog 5; Mothers against DPP homolog 5; MusMLP; SMA and MAD related protein 5; SMAD 5; SMAD family member 5; SMAD mothers against DPP homolog 5; Smad5; Smad5; SMAD5_HUMAN.
UNIPROT ID:   Q99717
Background:    Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. Smad5 is a receptor-regulated Smad (R-Smad). Smad5 is required for normal development of the cardiovascular system in vivo; lack of the Smad5 gene results in apoptosis of cardiac myocytes. 3 Upregulation of Smad5 has been reported to mediate apoptosis of gastric epithelial cells induced by Helicobacter pylori infection. Tissue specificity: Ubiquitous.
Immunogen:    Purified recombinant human SMAD5 (C-terminus) protein fragments expressed in E.coli.
Applications:    WB,ICC/IF,FC
Recommended Dilutions:    WB: 1/500-1/1000 IF: 1/50-1/200 FC: 1/50-1/100
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    4B10-B10-B6
MW:    Calculated MW: 52 kDa; Observed MW: 60 kDa
Isotype:    IgG1
Purification:    Affinity Purified
Species Reactivity:    Human
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunocytochemistry analysis of Smad5 in HeLa cells using Smad5 antibody.

Western blot analysis of SMAD5 (Cterminus) in Hela, Jurkat and K562 lysates using SMAD5 (Cterminus) antibody.

Flow Cytometry analysis of Jurkat cells stained with SMAD5 (red). Black line histogram represents the isotype control, normal mouse IgG.
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