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Smad2/3 (6F7) Mouse mAb

Anti-Smad2/3 (6F7) Mouse Monoclonal Antibody
5/5

$349.00

Cat.#:  N261294

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N261294
Product Name:   Anti-Smad2/3 (6F7) Mouse Monoclonal Antibody
Synonyms:   SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
UNIPROT ID:   P84022/Q15796
Background:    Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 . Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses .
Immunogen:    Recombinant Protein of Smad3
Applications:    IHC-P,WB
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:   
MW:    Calculated MW: 52 kDa; Observed MW: 52,60 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Rat,Mouse
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemical analysis of paraffin-embedded Human tonsils using Smad2/3 (6F7) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunohistochemistry analysis of paraffin-embedded Human Live rTissue using Smad2/3 (6F7) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of Smad2/3 (6F7) in C6, MCF-7, Jurkat and 3T3 lysates using Smad2/3 (6F7) antibody
          Publications