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cAMP Protein Kinase Catalytic Subunit Rabbit pAb

Anti-cAMP Protein Kinase Catalytic Subunit Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  N225097

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N225097
Product Name:   Anti-cAMP Protein Kinase Catalytic Subunit Rabbit pAb
Synonyms:   PRKACA; PKACA; cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; PRKACB; cAMP-dependent protein kinase catalytic subunit beta; PKA C-beta
UNIPROT ID:   P17612/P22694/P22612
Background:    PRKACA (protein kinase cAMP-activated catalytic subunit alpha) encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing’s syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms.
Immunogen:    The antiserum was produced against synthesized peptide derived from human PKA alpha/beta CAT. AA range:166-215
Applications:    WB,IHC-F,IHC-P,ICC/IF,ELISA
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Clone ID:   
MW:    Calculated MW: 40 kDa; Observed MW: 40 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Western blot analysis of cAMP Protein Kinase Catalytic Subunit in COLO205 lysates using PKA???¤/????/???? cat antibody.

Western blot analysis of cAMP Protein Kinase Catalytic Subunit in various lysates using cAMP Protein Kinase Catalytic Subunit antibody.
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