NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$349.00
Cat.#: N225149 |
Product Name: Anti-Phospho-FOXO1/3 (Ser322/S325) Rabbit pAb |
Synonyms: FOXO1; FKHR; FOXO1A; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; FOXO3; FKHRL1; FOXO3A; Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1 |
UNIPROT ID: Q12778/O43524 |
Background: Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. |
Immunogen: The antiserum was produced against synthesized peptide derived from human FOXO1A/3A around the phosphorylation site of Ser322 and Ser325. AA range:291-340 |
Applications: WB,IHC-P,ELISA |
Recommended Dilutions: WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000 |
Host Species: Rabbit |
Clonality: Rabbit Polyclonal |
Clone ID: – |
MW: Calculated MW: 70 kDa; Observed MW: 97 kDa |
Isotype: IgG |
Purification: Affinity Purified |
Species Reactivity: Human,Mouse,Rat |
Conjugation: Unconjugated |
Modification: Phosphorylated |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
Research Areas: Epigenetics and Nuclear Signaling |
Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing |
Western blot analysis of Phospho-FOXO1/3 (Ser322/S325) in 3T3 lysates using Phospho-FOXO1/3 (Ser322/S325) antibody. |