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Phospho-Estrogen Receptor alpha (Ser118) Rabbit pAb

Anti-Phospho-Estrogen Receptor alpha (Ser118) Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  N225290

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N225290
Product Name:   Anti-Phospho-Estrogen Receptor alpha (Ser118) Rabbit pAb
Synonyms:   ESR1; Era; Eralpha; Estrogen receptor; Estradiol receptor; ER-alpha; Estrogen receptor 1; NR3A1; ER; ESR; ESRA; Estrogen receptor alpha
UNIPROT ID:   P03372
Background:    ER (estrogen receptor 1) a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ER regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery. Phosphorylation provides an important mechanism to regulate ER activity. ER is phosphorylated on multiple sites.
Immunogen:    Synthetic peptide of human ESR1
Applications:    WB,IHC-P,ICC/IF
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 ICC: 1/100-1/200
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Clone ID:   
MW:    Calculated MW: 66 kDa; Observed MW: 66 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human
Conjugation:    Unconjugated
Modification:    Phosphorylated
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue using Estrogen Receptora(Phospho-Ser118) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. Sample with blocking peptide on the right.

Western blot analysis of Phospho-Estrogen Receptor alpha (Ser118) in MDAMB435 lysates using Phospho-Estrogen Receptor alpha (Ser118) antibody.

Immunofluorescence analysis of Phospho-Estrogen Receptor alpha (Ser118) in MCF-7 cells using Estrogen Receptora(Phospho-Ser118) antibody(red).
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