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HuB/HuD Rabbit pAb

Anti-HuB/HuD Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  N225369

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N225369
Product Name:   Anti-HuB/HuD Rabbit pAb
Synonyms:   ELAVL2; HUB; ELAV-like protein 2; ELAV-like neuronal protein 1; Hu-antigen B; HuB; Nervous system-specific RNA-binding protein Hel-N1; ELAVL4; HUD; PNEM; ELAV-like protein 4; Hu-antigen D; HuD; Paraneoplastic encephalomyelitis antigen HuD
UNIPROT ID:   Q12926/P26378
Background:    Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al. 1996 [PubMed 8663286]).
Immunogen:    The antiserum was produced against synthesized peptide derived from human ELAV2/4. AA range:61-110
Applications:    WB,IHC-F,IHC-P,ICC/IF,ELISA
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Clone ID:   
MW:    Calculated MW: 40 kDa; Observed MW: 40 kDa
Isotype:    IgG
Purification:    Affinity Chromatography
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunofluorescence analysis of HuB/HuD in HepG2 using ELAV2/4 antibody. The picture on the right is blocked using the synthesized peptide.

Western blot analysis of HuB/HuD in Hela lysates using HuB/HuD antibody.

Western blot analysis of HuB/HuD in Jurkat and HUVEC lysates using ELAV2/4 antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of HuB/HuD in 293 lysates using HuB/HuD antibody
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