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Cleaved-Caspase 6 p18 Rabbit pAb

Anti-Cleaved-Caspase 6 p18 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  N225413

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N225413
Product Name:   Anti-Cleaved-Caspase 6 p18 Rabbit pAb
Synonyms:   CASP6; MCH2; Caspase-6; CASP-6; Apoptotic protease Mch-2
UNIPROT ID:   P55212
Background:    Caspase-6 (Mch2) is one of the major executioner caspases functioning in cellular apoptotic processes. Upon apoptotic stimulation, initiator caspases such as caspase-9 are cleaved and activated. The activated upstream caspases further process downstream executioner caspases, such as caspase-3 and caspase-6, by cleaving them into large and small subunits, thereby initiating a caspase cascade leading to apoptosis. One of the major targets for caspase-6 is the membrane associated protein lamin A.
Immunogen:    The antiserum was produced against synthesized peptide derived from human Caspase 6. AA range:130-179
Applications:    WB,IHC-P,ELISA
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Clone ID:   
MW:    Calculated MW: 33 kDa; Observed MW: 20,35 kDa
Isotype:    IgG
Purification:    Affinity Chromatography
Species Reactivity:    Human,Rat
Conjugation:    Unconjugated
Modification:    Cleaved
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    apoptosis – Caspase family
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human lung carcinoma tissue using Caspase 6 (Cleaved-Asp16, 2) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. Sample with blocking peptide on the right.

Western blot analysis of Cleaved-Caspase 6 p18 in various lysates using Cleaved-Caspase 6 p18 antibody.

Western blot analysis of Cleaved-Caspase 6 p18 in 293 lysates using Cleaved-Caspase6 p18 (D179) antibody.

Western blot analysis of Cleaved-Caspase 6 p18 in 293 lysates treated with Etoposide using Cleaved-Caspase 6 p18 antibody.The lane on the right is blocked with the synthesized peptide.
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