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Claudin 1 Rabbit pAb

Anti-Claudin 1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  N225436

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N225436
Product Name:   Anti-Claudin 1 Rabbit pAb
Synonyms:   CLDN1; CLD1; SEMP1; Claudin-1; Senescence-associated epithelial membrane protein
UNIPROT ID:   O95832
Background:    The claudin family is composed of 23 integral membrane proteins, and their expression, which varies among tissue types, may determine both the strength and properties of the epithelial barrier. Alteration in claudin protein expression pattern is associated with several types of cancer. Claudin-1 is expressed primarily in keratinocytes and normal mammary epithelial cells, but is absent or reduced in breast carcinomas and breast cancer cell lines.
Immunogen:    The antiserum was produced against synthesized peptide derived from human Claudin 1. AA range:162-211
Applications:    WB,IHC-F,IHC-P,ICC/IF,ELISA
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Clone ID:   
MW:    Calculated MW: 23 kDa; Observed MW: 23 kDa
Isotype:    IgG
Purification:    Affinity Chromatography
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue, using Claudin 1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. Sample with blocking peptide on the right.

Western blot analysis of Claudin 1 in Hela lysates using Claudin 1 antibody.

Western blot analysis of Claudin 1 in HeLa lysates using Claudin 1 antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded Mouse Colon tissue, using Claudin 1 antibody.High-pressure and temperature Tris-EDTA pH 9.0 was used for antigen retrieval.
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