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Phospho-B23 (Thr199) Rabbit pAb

Anti-Phospho-B23 (Thr199) Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  N225495

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N225495
Product Name:   Anti-Phospho-B23 (Thr199) Rabbit pAb
Synonyms:   B23; NPM
UNIPROT ID:   P06748
Background:    Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486). In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029).
Immunogen:    The antiserum was produced against synthesized peptide derived from human NPM around the phosphorylation site of Thr199. AA range:171-220
Applications:    WB,IHC-F,IHC-P,ICC/IF,ELISA
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Clone ID:   
MW:    Calculated MW: 33 kDa; Observed MW: 33 kDa
Isotype:    IgG
Purification:    Affinity Chromatography
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Phosphorylated
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunofluorescence analysis of Phospho-B23 (Thr199) in HeLa treated with EGF using Phospho-B23 (Thr199) antibody. The picture on the right is blocked with the Phospho- peptide.

EnzymeLinked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phospho-peptide (Phospho-left) and NonPhospho-peptide (Phospho-right), using NPM (Phospho-Thr19antibody

Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma using Phospho-B23 (Thr199) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Sample with blocking peptide on the right.

Western blot analysis of Phospho-B23 (Thr199) in HepG2 lysates using Phospho-B23 (Thr199) antibody.The lane on the right is blocked with the synthesized peptide.
          Publications