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PMS2 Rabbit pAb

Anti-PMS2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  N225564

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N225564
Product Name:   Anti-PMS2 Rabbit pAb
Synonyms:   DNA mismatch repair gene; DNA mismatch repair protein PMS2; HNPCC4; PMS1 protein homolog 2
UNIPROT ID:   P54278
Background:    Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected.
Immunogen:    A synthesized peptide derived from human PMS2
Applications:    WB,IHC-P,ICC/IF,IP,FC
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 FC: 1/50-1/100
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Clone ID:   
MW:    Calculated MW: 96 kDa; Observed MW: 110 kDa
Isotype:    IgG
Purification:    Affinity Chromatography
Species Reactivity:    Human
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human tonsil using PMS2 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of PMS2 in (1) Jurkat lysates; (2)HeLa lysates using PMS2 antibody.
          Publications