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GRP78 BiP (4F11) Mouse mAb

Anti-GRP78 BiP (4F11) Mouse Monoclonal Antibody
5/5

$349.00

Cat.#:  N261400

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N261400
Product Name:   Anti-GRP78 BiP (4F11) Mouse Monoclonal Antibody
Synonyms:   HSPA5; GRP78; 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP
UNIPROT ID:   P11021
Background:    When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) (PubMed 8020977) pointed out that one of these, GRP78 (HSPA5), also referred to as ‘immunoglobulin heavy chain-binding protein’ (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).
Immunogen:    Purified recombinant human BiP/GRP78 protein expressed in E.coli.
Applications:    WB,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    4F11-D5-C10
MW:    Calculated MW: 72 kDa; Observed MW: 78 kDa
Isotype:    IgG2b
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    NeuroscienceyyyyyPrion disease
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunofluorescence analysis of GRP78 BiP (4F11) in Hela using BiP/GRP78 antibody.

Western blot analysis of GRP78 BiP (4F11) in 3T3, A549, C6 and Hela lysates using BiP/GRP78 antibody.

Immunoprecipitation analysis of GRP78 BiP (4F11) in Hela lysates using BiP/GRP78 antibody.Western blot analysis of BiP/GRP78 using BiP/GRP78 antibody.
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