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AKT Mouse mAb

Anti-AKT Mouse Monoclonal Antibody
5/5

$349.00

Cat.#:  N261403

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N261403
Product Name:   Anti-AKT Mouse Monoclonal Antibody
Synonyms:   AKT1
UNIPROT ID:   P31749/P31751/Q9Y243
Background:    Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.
Immunogen:    Purified recombinant human AKT1 protein fragments expressed in E.coli.AKT1 interacts (via the C-terminus) with CCDC88A (via its C-terminus). Interacts with GRB10; the interaction leads to GRB10 phosphorylation thus promoting YWHAE-binding By similarity. Interacts with AGAP2 (isoform 2/PIKE-A); the interaction occurs in the presence of guanine nucleotides. Interacts with AKTIP. Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. Interacts with CDKN1B; the interaction phosphorylates CDKN1B promoting 14-3-3 binding and cell-cycle progression. Interacts with MAP3K5 and TRAF6. Interacts with BAD, PPP2R5B, STK3 and STK4. Interacts (via PH domain) with SIRT1. Interacts with SRPK2 in a phosphorylation-dependent manner. Interacts with RAF1. Interacts with TRIM13; the interaction ubiquitinates AKT1 leading to its proteasomal degradation. Interacts with TNK2 and CLK2. Interacts (via the C-terminus) with THEM4 (via its C-terminus). Interacts with and phosphorylated by PDPK1.AKT2 interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. Interacts with CLK2, PBH2 and TRAF6. AKT3 interacts (via PH domain) with TCL1A; this enhances AKT3 phosphorylation and activation. Interacts with TRAF6.
Applications:    WB,IP
Recommended Dilutions:    WB: 1/500-1/1000 IP: 1/20
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    3B11-G8-B1
MW:    Calculated MW: 56 kDa; Observed MW: 60 kDa
Isotype:    IgG1
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunoprecipitation analysis of AKT in Hela lysates using AKT(pan) antibody.

Western blot analysis of total AKT in 3T3, 293T, PC-12 and K562 lysates using AKT(pan) antibody.

Western blot analysis of AKT1, AKT2, AKT3 and DSBA recombinant antigen using DSBA antibody,and(Right)AKT1, AKT2 and AKT3 recombinant antigen fragments using AKT(pan) antibody.
          Publications