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Histone H2A.X Rabbit mAb

Anti-Histone H2A.X Rabbit Monoclonal Antibody
5/5

$349.00

Cat.#:  N262330

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N262330
Product Name:   Anti-Histone H2A.X Rabbit Monoclonal Antibody
Synonyms:   H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X
UNIPROT ID:   P16104
Background:    Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Immunogen:    A synthetic peptide of human Histone H2A.X
Applications:    WB,IHC-F,IHC-P,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R02-5E6
MW:    Calculated MW: 15 kDa; Observed MW: 15 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Western blot analysis of Histone H2A.X in 3T3, Hela lysates using Histone H2A.X antibody

Immunocytochemistry analysis of Histone H2A.X (green) in HL-60 using Histone H2A.X antibody,and DAPI(blue).

Western blot analysis of Histone H2A.X in PC-12 lysates using Histone H2A.X antibody.

Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Histone H2A.X antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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