NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$495.00
Anti-Gα13GTP Mouse Monoclonal Antibody |
Cat. #: 26902 |
Size: 30 µL |
Gene Symbol: Gna13 |
Description: Anti-Gα13-GTP Mouse Monoclonal Antibody |
Background: Heterotrimeric G proteins are essential cellular signal transducers. Gα13 is one of the G proteins that could mediate cell migration and angiogenesis. Other biochemical and physiological functions of Gα13 are being explored. |
Immunogen: Recombinant full length active Gα13 protein |
Applications: IP, IHC and IF (Not applicable for WB since SDS denatures Gα13 GTPase) |
Published Applications: IF, IHC and IP – Click for Details |
Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
Concentration: 1 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG2b |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Anti-active Gα13 monoclonal antibody recognizes active Gα13 of vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Immunoprecipitation/Western blot: IP/WB analysis of active Gα13 proteins. Purified Gα13 proteins were treated with GDP (lane 1) or GTPGTPγS (lane 2). After immunoprecipitation with anti-active Gα13-GTP mouse monoclonal antibody (Cat. # 26902), these proteins were separated by SDS/PAGE and western blotted with anti- Gα13 mouse monoclonal antibody (Cat. # 26004). |
Immunohistochemistry: Staining of MEF cells (treated with PDGF) with anti- Gα13-GTP monoclonal antibody showing the localization of Gα13-GTP in the leading edge of migrating cells. |
1. Elabela-APJ axis attenuates cerebral ischemia/reperfusion injury by inhibiting neuronal ferroptosis Free Radic Biol Med. 2023 PMID: 36681202 |
2. The GABA B receptor mediates neuroprotection by coupling to G 13 Sci Signal. 2021 PMID: 34665640 |
3. Sphingosine 1-phosphate (S1P) suppresses the collagen-induced activation of human platelets via S1P4 receptor Thromb Res. 2017 PMID: 28609704 |
4. Resistance to inhibitors of cholinesterase-8A (Ric-8A) is critical for growth factor receptor-induced actin cytoskeletal reorganization J Biol Chem. 2011 PMID: 21771786 |
5. Agonist activation of the G protein-coupled receptor GPR35 involves transmembrane domain III and is transduced via Galpha and beta-arrestin-2 Br J Pharmacol. 2011 PMID: 20958291 |
6. Ga12/13 inhibition enhances the anti-cancer effect of bortezomib through PSMB5 downregulation Carcinogenesis. 2010 PMID: 20478922 |
7. Suppression of tumor angiogenesis by Galpha(13) haploinsufficiency J Biol Chem. 2009 PMID: 19654325 |