Pioneering GTPase and Oncogene Product Development since 2010

Email us: info@neweastbio.com

Call us: 6109452007

Human ADORA2A Full Length Protein

Human ADORA2A Full Length Protein-Synthetic Nanodisc
5/5

$4,400.00

Cat.#:  11005

   Size:   50 μg

In Stock

            Product Description          
Cat.#:     11005
Product Name:   Human ADORA2A Full Length Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   A2aR; ADORA2; RDC8
Target:    ADORA2A
UNIPROT ID:    P29274
Description:    Human ADORA2A full length protein-synthetic nanodisc
Background:     A member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein, an adenosine receptor of A2A subtype, uses adenosine as the preferred endogenous agonist and preferentially interacts with the G(s) and G(olf) family of G proteins to increase intracellular cAMP levels. It plays an important role in many biological functions, such as cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. It has been implicated in pathophysiological conditions such as inflammatory diseases and neurodegenerative disorders. Alternative splicing results in multiple transcript variants. A read-through transcript composed of the upstream SPECC1L (sperm antigen with calponin homology and coiled-coil domains 1-like) and ADORA2A (adenosine A2a receptor) gene sequence has been identified, but it is thought to be non-coding.
Species/Host:    HEK293
Molecular Weight:    The human full length ADORA2A protein has a MW of 44.7 kDa
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure1. Elisa plates were pre-coated with Flag Tag AODRA2A-Nanodisc (0.2 µg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with ADORA2A-Nanodisc is 1.181ng/ml.

Figure2. Human ADORA2A-Nanodisc, Flag Tag on SDS-PAGE
          Publications