NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$349.00
Cat.#: S222461 |
Product Name: Anti-6?His Tag Rabbit Polyclonal Antibody |
Synonyms: |
UNIPROT ID: |
Background: The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification. It can then be purified using immobilized metal ions, traditionally nickel, in a procedure termed immobilized-metal affinity chromatography. Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting. The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells. |
Immunogen: Synthetic peptide: 18?His conjugated to KLH |
Applications: WB |
Recommended Dilutions: WB: 2000-10000; |
Host Species: Rabbit |
Clonality: Rabbit Polyclonal |
Isotype: Immunogen-specific rabbit IgG |
Purification: Antigen affinity purification |
Species Reactivity: Fusion proteins containing the HIS Tag |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol |
Research Areas: Cell Biology |
Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing |
Gel: 8%SDS-PAGE, Lysate: 0.5/0.25/0.5/0.25 μg; Lane 1-4: 0.5/0.25/0.5/0.25 μg Fusion protein containing the HIS Tag; Primary antibody: 222461(6?His Tag Antibody) at dilution 1/2000; Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution; Exposure time: 5 seconds |