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6XHis Tag Rabbit pAb

Anti-6XHis Tag Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S222461

   Size:   100 μl

In Stock

            Product Description          
Cat.#:   S222461
Product Name:   Anti-6?His Tag Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   
Background:    The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification. It can then be purified using immobilized metal ions, traditionally nickel, in a procedure termed immobilized-metal affinity chromatography. Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting. The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.
Immunogen:    Synthetic peptide: 18?His conjugated to KLH
Applications:    WB
Recommended Dilutions:    WB: 2000-10000;
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Fusion proteins containing the HIS Tag
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Gel: 8%SDS-PAGE, Lysate: 0.5/0.25/0.5/0.25 μg;
Lane 1-4: 0.5/0.25/0.5/0.25 μg Fusion protein containing the HIS Tag;
Primary antibody: 222461(6?His Tag Antibody) at dilution 1/2000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 5 seconds
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