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ABCB5 Rabbit pAb

Anti-ABCB5 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216315

   Size:   100 μl

In Stock

            Product Description          
Cat.#:   S216315
Product Name:   Anti-ABCB5 Rabbit Polyclonal Antibody
Synonyms:   ABCB5beta; EST422562; ABCB5alpha
UNIPROT ID:   Q2M3G0 (Gene Accession – BC104894 )
Background:    ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).
Immunogen:    Fusion protein of human ABCB5
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 216315(ABCB5 Antibody) at a dilution of 1/70(Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 216315(Anti-ABCB5 Antibody) at dilution 1/70.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 216315(Anti-ABCB5 Antibody) at a dilution of 1/70.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D220189(Anti-ABCB5 Antibody) at dilution 1/70.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: 293T cell lysate;
Primary antibody: 216315(ABCB5 Antibody) at dilution 1/600;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds
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