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ABHD2 Rabbit pAb

Anti-ABHD2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221301

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221301
Product Name:   Anti-ABHD2 Rabbit Polyclonal Antibody
Synonyms:   HS1-2; LABH2; PHPS1-2
UNIPROT ID:   P08910 (Gene Accession – NP_008942 )
Background:    This gene encodes a protein containing an alpha/beta hydrolase fold, which is a catalytic domain found in a wide range of enzymes. The encoded protein is an acylglycerol lipase that catalyzes the hydrolysis of endocannabinoid arachidonoylglycerol from the cell membrane. This leads to activation of the sperm calcium channel CatSper, which results in sperm activation. Alternative splicing of this gene results in two transcript variants encoding the same protein.
Immunogen:    Synthetic peptide of human ABHD2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 221301(ABHD2 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 221301(Anti-ABHD2 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 221301(Anti-ABHD2 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with synthetic peptide and then with D262830(Anti-ABHD2 Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: HT-29 and HepG2 cell lysates;
Primary antibody: 221301(ABHD2 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds
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