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ABL2 Rabbit pAb

Anti-ABL2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220029

   Size:   100 μl

In Stock

            Product Description          
Cat.#:   S220029
Product Name:   Anti-ABL2 Rabbit Polyclonal Antibody
Synonyms:   ARG; ABLL
UNIPROT ID:   P42684 (Gene Accession – NP_001129472 )
Background:    This gene encodes a member of the Abelson family of nonreceptor tyrosine protein kinases. The protein is highly similar to the c-abl oncogene 1 protein, including the tyrosine kinase, SH2 and SH3 domains, and it plays a role in cytoskeletal rearrangements through its C-terminal F-actin- and microtubule-binding sequences. This gene is expressed in both normal and tumor cells, and is involved in translocation with the ets variant 6 gene in leukemia. Multiple alternatively spliced transcript variants encoding different protein isoforms have been found for this gene.
Immunogen:    Synthetic peptide of human ABL2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 220029(ABL2 Antibody) at a dilution of 1/25(Cytoplasm ).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 220029(Anti-ABL2 Antibody) at dilution 1/25.

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 220029(Anti-ABL2 Antibody) at a dilution of 1/25.

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with synthetic peptide and then with D260816(Anti-ABL2 Antibody) at dilution 1/25.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela cells, Jurkat cells;
Primary antibody: 220029(ABL2 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 minutes
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