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ACSL4 Rabbit pAb

Anti-ACSL4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217090

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217090
Product Name:   Anti-ACSL4 Rabbit Polyclonal Antibody
Synonyms:   ACS4; FACL4; LACS4; MRX63; MRX68; XLID63
UNIPROT ID:   O60488 (Gene Accession – BC034959 )
Background:    The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the cognitive disability or Alport syndrome. Alternative splicing of this gene generates multiple transcript variants.
Immunogen:    Fusion protein of human ACSL4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 217090(ACSL4 Antibody) at a dilution of 1/75(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 217090(Anti-ACSL4 Antibody) at dilution 1/75.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: RAW264.7, MCF7 cell, Mouse brain tissue, 293T cell, HepG2 cell lysates;
Primary antibody: 217090(ACSL4 Antibody) at dilution 1/1300;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds
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