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ACTA2 Rabbit pAb

Anti-ACTA2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220363

   Size:   100 μl

In Stock

            Product Description          
Cat.#:   S220363
Product Name:   Anti-ACTA2 Rabbit Polyclonal Antibody
Synonyms:   ACTSA
UNIPROT ID:   P62736 (Gene Accession – NP_001135417 )
Background:    This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome.
Immunogen:    Synthetic peptide of human ACTA2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cardiovascular, Stem Cells
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 220363(ACTA2 Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the synthetic peptide and then with 220363(Anti-ACTA2 Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 220363(Anti-ACTA2 Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with synthetic peptide and then with D261431(Anti-ACTA2 Antibody) at dilution 1/40.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: NIH/3T3 cell, C2C12 cell lysates;
Primary antibody: 220363(ACTA2 Antibody) at dilution 1/600;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 10 seconds
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