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ACTB Rabbit pAb

Anti-ACTB Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  22077

   Size:   100 μl

In Stock

          Product Description          
Cat.#:  22077
Product Name:   Anti-ACTB Rabbit Polyclonal Antibody
Synonyms:   BRWS1; PS1TP5BP1
UNIPROT ID:   P60709 (Gene Accession – NP_001092 )
Background:    This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Immunogen:    Synthetic peptide of human ACTB
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Isotype/Loading Controls, Tags & Cell Markers, Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing
Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 210012(Anti-ACTB Antibody) at a dilution of 1/40(Cytoplasm ). In comparison with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the synthetic peptide and then with 210012(Anti-ACTB Antibody) at dilution 1/40.
Gel: 8%SDS-PAGE, Lysate: 40 μg; Lane 1-7: NIH/3T3 cell, Jurkat cell, 231 cell, LOVO cell, Mouse kidney tissue, HepG2 cell, HT29 cell lysates; Primary antibody: 210012(ACTB Antibody) at dilution 1/2000; Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution; Exposure time: 1 minute
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