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ACTL6B Rabbit pAb

Anti-ACTL6B Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219116

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219116
Product Name:   Anti-ACTL6B Rabbit Polyclonal Antibody
Synonyms:   ACTL6; BAF53B; arpNalpha
UNIPROT ID:   O94805 (Gene Accession – BC020944 )
Background:    The protein encoded by this gene is a member of a family of actin-related proteins (ARPs) which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. This subunit may be involved in the regulation of genes by structural modulation of their chromatin, specifically in the brain. Alternative splicing results in multiple transcript variants.
Immunogen:    Fusion protein of human ACTL6B
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-300;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Neuroscience, Stem Cells
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 219116(ACTL6B Antibody) at a dilution of 1/100(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 219116(Anti-ACTL6B Antibody) at dilution 1/100.

The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 219116(Anti-ACTL6B Antibody) at a dilution of 1/100.

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with fusion protein and then with D225871(Anti-ACTL6B Antibody) at dilution 1/100.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela and HepG2 cell lysates;
Primary antibody: 219116(ACTL6B Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 10 seconds
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