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ACTN1 Rabbit pAb

Anti-ACTN1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216987

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216987
Product Name:   Anti-ACTN1 Rabbit Polyclonal Antibody
Synonyms:   BDPLT15
UNIPROT ID:   P12814 (Gene Accession – BC003576 )
Background:    Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Fusion protein of human ACTN1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 15-50;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 216987(ACTN1 Antibody) at a dilution of 1/10(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 216987(Anti-ACTN1 Antibody) at dilution 1/10.

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 216987(Anti-ACTN1 Antibody) at a dilution of 1/10.

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with fusion protein and then with D221591(Anti-ACTN1 Antibody) at dilution 1/10.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: 231, A549 and Hela cell, Human fetal muscle tissue lysates;
Primary antibody: 216987(ACTN1 Antibody) at dilution 1/500;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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