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ADAM15 Rabbit pAb

Anti-ADAM15 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216938

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216938
Product Name:   Anti-ADAM15 Rabbit Polyclonal Antibody
Synonyms:   MDC15
UNIPROT ID:   Q13444 (Gene Accession – BC014566 )
Background:    The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.
Immunogen:    Fusion protein of human ADAM15
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human tonsil tissue using 216938(ADAM15 Antibody) at a dilution of 1/70(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human tonsil tissue is first treated with the fusion protein and then with 216938(Anti-ADAM15 Antibody) at dilution 1/70.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: NIH/3T3, HepG2, PC-3, Jurkat cell, Mouse heart tissue lysates;
Primary antibody: 216938(ADAM15 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 5 minutes
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