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ADRA1B Rabbit pAb

Anti-ADRA1B Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220289

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220289
Product Name:   Anti-ADRA1B Rabbit Polyclonal Antibody
Synonyms:   ADRA1; ALPHA1BAR
UNIPROT ID:   P35368 (Gene Accession – NP_000670 )
Background:    Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1B-adrenergic receptor, which induces neoplastic transformation when transfected into NIH 3T3 fibroblasts and other cell lines. Thus, this normal cellular gene is identified as a protooncogene. This gene comprises 2 exons and a single large intron of at least 20 kb that interrupts the coding region.
Immunogen:    Synthetic peptide of human ADRA1B
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 1000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 220289(ADRA1B Antibody) at a dilution of 1/25(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 220289(Anti-ADRA1B Antibody) at dilution 1/25.

The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 220289(Anti-ADRA1B Antibody) at a dilution of 1/25.

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with synthetic peptide and then with D261268(Anti-ADRA1B Antibody) at dilution 1/25.

Gel: 8%SDS-PAGE, Lysate: 80 μg;
Lane 1-3: 293T cells, PC3 cells, hela cells;
Primary antibody: 220289(ADRA1B Antibody) at dilution 1/500;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 minutes
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