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AK2 Rabbit pAb

Anti-AK2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217129

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217129
Product Name:   Anti-AK2 Rabbit Polyclonal Antibody
Synonyms:   ADK2; AK 2
UNIPROT ID:   P54819 (Gene Accession – BC009405 )
Background:    Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Mutations in this gene are the cause of reticular dysgenesis. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1 and 2.
Immunogen:    Fusion protein of human AK2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 217129(AK2 Antibody) at a dilution of 1/30(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 217129(Anti-AK2 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 217129(Anti-AK2 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D221850(Anti-AK2 Antibody) at dilution 1/30.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-7: Human placenta tissue, A549 cells, mouse brain tissue, hepG2 cells, Raji cells, human fetal liver tissue, hela cells;
Primary antibody: 217129(AK2 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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