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AKR1C4 Rabbit pAb

Anti-AKR1C4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217519

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217519
Product Name:   Anti-AKR1C4 Rabbit Polyclonal Antibody
Synonyms:   C11; CDR; DD4; CHDR; DD-4; HAKRA; 3-alpha-HSD
UNIPROT ID:   P17516 (Gene Accession – BC020744 )
Background:    This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.
Immunogen:    Full length fusion protein
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 217519(AKR1C4 Antibody) at a dilution of 1/25(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 217519(Anti-AKR1C4 Antibody) at dilution 1/25.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: A549 cell lysate and Human fetal liver tissue lysate;
Primary antibody: 217519(AKR1C4 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 seconds
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